Influence of protein corona on the interaction of glycogen–siRNA constructs with ex vivo human blood immune cells

M Wojnilowicz; P Laznickova; Y Ju; CS Ang; F Tidu; K Bendickova; G Forte; M Plebanski; F Caruso; F Cavalieri; J Fric

Journal article

Influence of protein corona on the interaction of glycogen–siRNA constructs with ex vivo human blood immune cells

M Wojnilowicz, P Laznickova, Y Ju, CS Ang, F Tidu, K Bendickova, G Forte, M Plebanski, F Caruso, F Cavalieri, J Fric

Biomaterials Advances | ELSEVIER | Published : 2022

DOI: 10.1016/j.bioadv.2022.213083

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Abstract

Glycogen–nucleic acid constructs i.e., glycoplexes are emerging promising platforms for the alteration of gene expression and transcription. Understanding the interaction of glycoplexes with human blood components, such as serum proteins and peripheral blood mononuclear cells (PBMCs), is important to overcome immune cell activation and control biodistribution upon administration of the glycoplexes in vivo. Herein, we investigated the interactions of polyethylene glycol (PEG)ylated and non-PEGylated glycoplexes carrying siRNA molecules with PBMCs isolated from the blood of healthy donors. We found that both types of glycoplexes were non-toxic and were primarily phagocytosed by monocytes witho..

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University of Melbourne Researchers

Yi David Ju Author

Ching-Seng Ang Author

Frank Caruso Author

Francesca Cavalieri Author

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Awarded by Ministerstvo Zdravotnictví Ceské Republiky

Funding Acknowledgements

This work was supported by a University of Melbourne Establishment Grant (F. Cavalieri and M.W.) . This work was funded by an Australian Research Council (ARC) Future Fellowship (FT140100873, F. Cavalieri) , a National Health and Medical Research Council (NHMRC) Senior Principal Research Fellowship (GNT1135806, F. Caruso) , and an NHMRC Senior Research Fellowship B (GNT1042059, M.P.) . This research was also supported by a European Social Fund and European Regional Development Fund-Project MAGNET (No. CZ.02.1.01/0.0/0.0/15_003/0000492) and project no. LQ1605 from the National Program of Sustainability II (MEYS CR) . The Ministry of Health of the Czech Republic-DRO (Institute of Hematology and Blood Transfusion - IHBT, 00023736) . This project received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 690901. This work was performed in part at the Materials Characterization and Fabrication Plat-form (MCFP) . The authors thank the technical support team at the Center for Translational Medicine (FNUSA-ICRC) for technical support and Insight Editing London for editing the manuscript prior to submission.